The study of the cell cycle focuses on mechanisms that regulate the timing and frequency of DNA duplication and cell division. As a biological concept, the cell cycle is defined as the period ...

Expression of the cell cycle inhibitor p15 INK4b is suppressed by the Myc oncoprotein and induced by transforming growth factor-β (TGF-β). The transcription factor Miz-1 activates the Ink4b ...

Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...

When looking at cells with a microscope, the length of different stages of the cell cycle can be estimated using the formula: \(\text{Length of time in phase}=\) \(\frac{\text{observed number of ...

Before a cell can divide, it must grow and make copies of all the organelles such as mitochondria and ribosomes. The cell must also replicate the chromosomes in the nucleus, then it can divide by ...

A life cycle is the series of stages something passes through during its lifetime. We also use the term for things that are not living, like cell phones. The life cycle for anything you buy includes ...

In tumoral cells, the modulation of the G1/S phases of cell cycle is destabilized by amplification and high expression of cyclin E (CCNE) or by mutation or loss of retinoblastoma 1 (RB1) gene. Cancer ...